Validated Signatures

Below is a list of signatures derived from Insignia and validate by various laboratory experiments.

Vibro cholerae O1 biovar El Tor strain N16961

Insignia was used to develop assays for the identification of V. cholerae at the species level using a TaqMan Real-Time qPCR format. The initial version of Insignia queried a database that was populated with ~300 bacterial genomes, including one strain of V. cholerae (O1 biovar El Tor strain N16961), and four near neighbors in the family Vibrionaceae (three Vibrio and one Photobacterium species). The Insignia Web interface was used to retrieve all 20-mers unique to V. cholerae, from which 50 TaqMan assays were designed.

Results:

• Assay signature sequences are provided in Table 1
• Inclusive and exclusive strain information in Table 2
• Detailed qPCR results for all 3,450 validation experiments in Table 3