Examples

The Insignia interface is designed to allow users run quick signature queries with ease. This page attempts to work the user through a simple example with Bacillus anthracis Ames and also give explanations on what the results signify. Lastly, this page gives an overview on how to navigate the Argo graphical representation of the query genome.

Running Insignia

The Insignia pipeline can be run by clicking the "Run Insignia" link on the left navigation panel. On the query page, the user can select one reference genome and optional one or more target genomes.

Possible Scenario:
If the user wishes to calculate all signatures that are common to Bacillus anthracis Ames and all other Bacillus strains, Bacillus anthracis Ames should be selected in the "Reference" genome list and then all Bacillus should be selected in the "Target" genomes list. No organism is selected in the "Background" genomes list. If no organism in the background is selected, Insignia will compare the reference and target genomes against every organism in the database. If an organism is selected in the background, Insignia will find signatures that are common to the user's reference and target genomes by comparing them only to the select background genome(s).
Lastly, the user can specify the signature length (>=18). By clicking "signify", the signatures will be calculated

Results

Insignia prints out a series of results when the signatures are calculated as shown below:

Target: Shows which organisms were chosen as your target genome(s)
Running: Shows what processes were run in the pipeline
Signature(s): This output of Insignia displays the calculated signatures

The output signatures can be viewed graphically. For a description of the graphical output, visit the help page